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Science articlesClinical and anamnestic features of children of early and preschool age with hypertrophy of the pharyngeal tonsil and local expression of DNA marker of Streptococcus pyogenes
E. V. Shabaldina (1), D. R. Akhtyamov (2), S. V. Gorshkova (1), N. S. Deeva (1), A. V. Shabaldin (1), (3), M. L. Filipenko (4)
(1) Kemerovo State Medical University, Ministry of Healthcare of Russia, Kemerovo, 650003, Russia
(2) Regional Children’s Clinical Hospital, Kemerovo, 650003, Russia
(3) Scientific Research Institute of Complex Problems of Cardio-Vascular Diseases, Kemerovo, 650002, Russia
(4) Institute of Chemical Biology and Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia
UDK: 616.322-007.61-053.1
DOI: https://doi.org/10.18692/1810-4800-2018-6-64-71
ABSTRACT
Streptococcus pyogenes is a cause of immune inflammatory diseases, such as rheumatic disease and acute post-streptococcal glomerulonephritis. The associations of Streptococcus pyogenes with chronic tonsillitis and hypertrophy of the tonsil are shown. The question of the association of the expression of Streptococcus pyogenes on the pharyngeal tonsil with its hypertrophy and hypertrophy of the tonsils remains open. The aim of the study was to study the clinical and anamnestic features of children of early and preschool age with hypertrophy of the pharyngeal tonsil and local expression of DNA markers of Streptococcus pyogenes. Surveyed 228 children in the age range of 2–6 years who underwent surgical treatment (adenotomy, adenotonsillotomy) in the children's otorhinolaryngological department of the Kemerovo Regional Children's Clinical Hospital. The mothers of these children responded to the questionnaire. All children underwent microdebrider adenotomy, and 55 children received additional tonsillotomy. A cell scraping was taken from the pharyngeal tonsil to isolate total DNA from the pharyngeal tonsil biotope. Polymerase chain reaction (PCR) was carried out in real time to identify such microorganisms as Streptococcus pyogenes (StrPyo), Streptococcus pneumoniae (StrPne), Streptococcus agalactiae (StrAgl), Staphylococcus aureus (Staph aur), family Streptococcaceae (Strep), family Staphylococcaceae (Staph), Cytomegalovirus (CMV) and Epstein-Barr Virus (EBV). A linear regression analysis was performed, where the main dependent factor was the expression level of StrPyo, and all factors of the questionnaire and the levels of DNA expression of markers of other microorganisms were independent factors. Linear regression analysis showed that the level of expression of the StrPyo marker DNA on the pharyngeal tonsil was positively associated with such indicators as the number of pregnancies in history, the degree of hypertrophy of the tonsils and the expression of the Streptococcaceae family on the pharyngeal tonsil DNA. DNA expression of the Streptococcus pyogenes marker on the pharyngeal tonsil indicates a more unfavorable course of the pathological process in the tonsils of the lymphoid pharyngeal ring and the risk of the formation of local and systemic immuno-inflammatory pathology.
Publication date:
06.12.2018
Keywords:
hypertrophy of tonsils of pharyngeal lymphoid ring, Streptococcus pyogenes, PCR. For citation:
Shabaldina E. V., Akhtyamov D. R., Gorshkova S. V., Deeva N. S., Shabaldin A. V., Filipenko M. L. Clinical and anamnestic features of children of early and preschool age with hypertrophy of the pharyngeal tonsil and local expression of DNA marker of Streptococcus pyogenes. Rossiiskaya otorinolaringologiya. 2018;6:64–71. https://doi.org/10.18692/1810-4800-2018-6-64-71