Section: 
Otiology
OtiologyImmunogenetic biomarkers in chronic suppurative otitis media in residents of Republic of Dagestan
Z. M. Saidov (1), Yu. A. Dzhamaludinov (2), Kh. M. Diab (3), V. Yu. Khanaliev (4), A. V. Krivtsov (5), M. A. Shakhnazarov (6), M. Z. Saidov (7)
(1), (2), (4), (5), (6), (7) Dagestan State Medical University, Makhachkala, 367000, Russian Federation, (3) Otorhinolaryngology Medical Research Center of the Federal Medico-Biological Agency, Moscow, 123182, Russian Federation
UDK: УДК 616.284-002.2:57.083.3(470.67)
DOI: https://doi.org/10.18692/1810-4800-2024-5-73-82
ABSTRACT
Abstract. The course and outcome of chronic suppurative otitis media (CSOM) depends on the reactivity of both local and systemic immunity, which in turn depends on the genes of the human major histocompatibility complex (MHC). Human MHC proteins belong to the human leukocyte antigens — HLA. Objective. To study associations of allelic variants of HLA genes as well as the frequencies of homozygotes of allelic variants of HLA genes with mesotympanitis and epitympanitis in residents of the Republic of Dagestan. Patients and methods. 38 patients with mesotympanitis and epitympanitis. The control group included 11 people. Genotyping for the DQA1, DQB1, and DRB1 loci of the HLA system was carried out by DNA amplification. The distribution of frequencies of alleles and homozygotes of the indicated HLA gene loci in CSOM in residents of the Republic of Dagestan was studied. Results. It was shown that with mesotympanitis in residents of the Republic of Dagestan there is a statistically significant increase in the frequency of the *02 allele of the DQB1 gene and the *07 allele of the DRB1 gene. At the same time, carriers of these alleles had a statistically significant increased relative risk of developing mesotympanitis. In epitympanitis, a statistically significant increase in the frequency of occurrence is observed for the *0102 allele of the DQA1 gene and for the *02 allele of the DQB1 gene. In carriers of these alleles, the relative risk of developing epitympanitis was also increased. A selective comparison of the distribution of frequencies of homozygotes for loci DQA1, DQВ1, DRВ1 among residents of the Republic of Dagestan showed that the number of associations for epitympanitis compared with mesotympanitis was more than 2 times greater: for epitympanitis, 19; and for mesotympanitis, 8. Conclusion. The study of immunogenetic biomarkers of the HLA system complements the pathogenetic aspects of CSOM, which opens up the possibility of choosing immunotropic therapy for patients with CSOM to improve the postoperative course of the disease and improve the prognosis. It is very promising to study the predictive power of HLA genotyping in relation to the outcomes of tympanoplasty in CSOM
Publication date:
17.10.2024
Keywords:
mesotympanitis, epitympanitis, histocompatibility genes, alleles, homozygotes For citation:
Saidov Z. M., Dzhamaludinov Yu. A., Diab Kh. M., Khanaliev V. Yu., Krivtsov A. V., Shakhnaza-rov M. A., Saidov M. Z. Immunogenetic biomarkers in chronic suppurative otitis media in residents of Republic of Dagestan. Russian otorhinolaryngology. 2024;23(5):73-82. (In Russ.) https://doi.org/10.18692/1810-4800-2024-5-73-82